AREX® Fluor 647 Mouse IgG (H+L) F(ab')2 Fragment Donkey Polyclonal Antibody
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Conjugate:
- Storage:
-
Brand:
CAT.NO. : ARA6332
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Size:
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Product Details
Background
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
|
Dilution |
1:100-1:500 |
Overview
|
Host Species |
Donkey |
|
Species Reactivity |
Mouse |
|
Physical State |
Liquid |
|
Purification Method |
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
|
Storage Buffer |
0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 7.6. |
|
Preservative |
0.01% Sodium Azide |
|
Fluorophore |
Amax=642nm, Emax=662nm |
|
Stabilizer |
5 mg/ml BSA |
|
Absorbance Ratio |
A651/A280 = 4.0 |
Data
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Actin antiboody and AREX® Fluor 647 Mouse IgG (H+L) F(ab')2 Fragment Donkey Polyclonal Antibody at dilution of 1:150.
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-human RBAP48 Monoclonal antibody and AREX® Fluor 647 Mouse IgG (H+L) F(ab')2 Fragment Donkey Polyclonal Antibody at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer .
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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