TRBC1 Mouse Monoclonal Antibody(ARA662)
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Gene Symbol:
- Storage:
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Brand:
CAT.NO. : ARA6380
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Product Details
Background
Constant region of T cell receptor (TR) alpha chain (PubMed:24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed:25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed:9382891, PubMed:23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed:15040585). [UniProt]
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IHC |
1:100 - 1:500 |
Overview
|
Product Description |
Mouse monoclonal to TRBC1 |
|
Immunogen |
Recombinant fusion protein of human TRBC1 expressed in E. Coli |
|
Purification Method |
This antibody is purified through a protein G column. |
|
Clonality |
Monoclonal |
|
Form |
Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
|
Gene Symbol |
TRBC1 |
|
Alternative Names |
T-cell receptor beta-1 chain C region |
|
Gene ID (Human) |
28639 |
|
Protein ID (Human) |
P01850 |
Data
Western blot analysis of TRBC1 expression in HUVEC (A), Jurkat (B), Hela (C), HUVE12 (D), A549 (E), C6 (F), Raji (G), T47D (H) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 40 kD kD)
Immunohistochemical analysis of TRBC1 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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