Thymidine Phosphorylase Mouse Monoclonal Antibody(ARA840)

Datasheet

MSDS

Thymidine Phosphorylase Mouse Monoclonal Antibody(ARA840)

Key features and details

Target: Thymidine Phosphorylase
Host: Mouse
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IHC
Storage: -20°C

Brand:

CAT.NO. : ARA6648

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Platelet-derived endothelial cell growth factor (PD-ECGF), which is alternatively designated thymidine phosphorylase or gliostatin, is an angiogenic inducer that potently stimulates the growth of endothelial cells and induces chemotaxis. Biologically active PD-ECGF is a functional dimer that consists of two single polypeptide chains that are expressed in platelets, placenta, foreskin fibroblasts and various squamous cell carcinomas, and they are slowly secreted from the cells. In addition, PD-ECGF is overexpressed in tumor and lesional psoriatic skin and lesional epidermis, indicating that it may play a role in the pathophysiology of psoriasis. Serine residues of PD-ECGF are frequently associated with nucleotide triphosphates, including ATP. In an ATP dependent manner, PD-ECGF is also able to catalyze the reversible phosphorolysis of thymidine to thymine, as it contains thymidine phosphorylase activities.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:800-1:1,000
IHC	1:3,000-1:10,000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Name	Thymidine Phosphorylase Recombinant Mouse Monoclonal Antibody (ARA840)
Antibody Type	Recombinant Mouse Monoclonal Antibody
Immunogen	Recombinant protein within Human Thymidine Phosphorylase aa 19-239 /482.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC-P
Molecular Weight	Predicted band size: 50 kDa
Positive Control	THP-1 cell lysate, A549 cell lysate, A31 cell lysate, HeLa cell lysate, mouse spleen tissue, rat spleen tissue.
Conjugation	unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Thymidine Phosphorylase Mouse Monoclonal Antibody(ARA840)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Thymidine Phosphorylase Mouse Monoclonal Antibody(ARA840)

Western blot analysis of Thymidine Phosphorylase on different lysates with Thymidine Phosphorylase Mouse Monoclonal Antibody(ARA840) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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