Human FADS2 ELISA Kit
MSDS 
Key features and details
- Sensitivity:2.6 pg/mL
- Detection Range:78.1-5000 pg/mL
- Sample Types: Cell lysate
- Species:Human
- Detection Method: Sandwich ELISA
- Target:FADS2
-
Brand:
CAT.NO. : ARE6644
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Product Details
Background
Human FADS2 ELISA Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FADS2 ELISA kit is to be used to detect and quantify protein levels of endogenous human FADS2. The assay recognizes human FADS2. An antibody specific for human FADS2 has been pre-coated onto the microwells. The human FADS2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FADS2 is added to detect the captured human FADS2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Overview
|
Product name |
Human FADS2 ELISA Kit |
|
Tests Quantity |
1 X 96 well plate |
|
Sample type |
Cell lysate |
|
Assay type |
Sandwich |
|
Sensitivity / LOD |
2.6 pg/mL |
|
Detection Range |
78.1-5000 pg/mL |
|
Species Reactivity |
Human |
|
Applications |
Sandwich ELISA |
|
Gene ID |
9415 (Human) |
|
Synonyms |
Acyl-CoA 6-desaturase, D6D, Delta-6 desaturase, EC:1.14.19.3 |
Data
Standard Curve
Performance
Recovery
IntraAssay
InterAssay
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 86%-95% |
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,475.2 | 113.2 | 4.6 |
| 2 | 8 | 589.4 | 49.7 | 8.4 |
| 3 | 8 | 322.1 | 31.0 | 9.6 |
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,553.7 | 140.4 | 5.5 |
| 2 | 16 | 596.7 | 51.5 | 8.6 |
| 3 | 16 | 333.5 | 33.9 | 10.2 |
Storage
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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