Ribosomal Protein L32 Rabbit Polyclonal Antibody
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to Ribosomal Protein L32 |
Specificity | Recognizes endogenous levels of Ribosomal Protein L32 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Ribosomal Protein L32. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 15 kD; Observed: 16 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | 60S ribosomal protein L32 |
Gene Symbol | RPL32 |
Entrez Gene | 6161(Human); 102642959; 19951(Mouse); 28298(Rat) |
SwissProt | P62910(Human); P62911(Mouse); P62912(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Ribosomal Protein L32 expression in HepG2 (A), NIH3T3 (B), mouse spleen (C), rat ovary (D) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 16 kD)
Immunohistochemical analysis of Ribosomal Protein L32 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Ribosomal Protein L32 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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