pTau205 Mouse Monoclonal Antibody(ARA990)

Datasheet

MSDS

pTau205 Mouse Monoclonal Antibody(ARA990)

Key features and details

Target: pTau205
Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Application: Sandwich ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6888

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

pTau205 (phosphorylated tau at position 205) is a developing biomarker for Alzheimer's disease (AD) specifically linked to the presence and advancement of mature tau neurofibrillary tangles (NFTs), a key feature of the disease.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
Sandwich ELISA	1: 100- 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody specificity	Raised against a peptide immunogen containing phosphorylated T205. The antibody recognizes the pTau protein with an epitope containing phosphorylated T205. The antibody also weakly reacts with phosphoryla
Isotype	IgG1
Immunogen	Human MAPT peptide
Cross reactivity	Not tested
Recombinant	Not recombinant
Purification	Protein A purification
Form/Buffer	PBS, carrier-free, with optional 0.09% sodium azide
Conjugation	Unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Sandwich ELISA analysis of pTau205 by ARA6887 & ARA6837. Microtiter plates were coated with ARA6887 as the capture antibody. Recommbinant pTau205 protein was applied as the antigen. Biotin-conjugated Tau (S305) Mouse Monoclonal Antibody(ARA971) served as the detection antibody, followed by streptavidin-HRP incubation. Result: ARA6887 and ARA6837 can be used as a matched antibody pair to detect and quantify the concentration of pTau205.

Formulation

ARA6888	Phosphate-buffered saline (PBS), carrier-free, with optional 0.09% sodium azide
ARA6888-T5/20/50/100	Phosphate-buffered saline (PBS) with 50% glycerol, 0.5% BSA and 0.09% sodium azide

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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