Antigen Retrieval Solution I (Citrate)
Key features and details
- Buffer Type: Citrate
- pH Range: 5.5–6.5
- Form: Ready-to-Use
- Applicable Sample: FFPE Tissue
- Storage & Shelf Life: 2–30°C, 18 months
- Compatibility: Manual & Automated IHC
- Batch Performance: Consistent Intra/Inter-batch
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Brand:
CAT.NO. : ARB0005
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Size:
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Product Details
Intended Use
For epitope retrieval prior to immunohistochemical staining.
Detection Principle
Tissues or cells fixed with paraformaldehyde, formaldehyde or other aldehyde reagents will form cross-links between proteins, masking antigen epitopes and weakening staining signals during immunoassay.
Heat treatment of tissue sections in epitope retrieval buffer can restore masked antigen epitopes, break cross-linked peptide chains without destroying epitope structures. It enhances specific staining intensity, reduces non-specific background, and lowers false positive and false negative results.
Heat treatment of tissue sections in epitope retrieval buffer can restore masked antigen epitopes, break cross-linked peptide chains without destroying epitope structures. It enhances specific staining intensity, reduces non-specific background, and lowers false positive and false negative results.
Main Components
Trisodium citrate, citric acid, surfactant, etc.
Other required reagents & materials not supplied:
Wash buffer, deparaffinization solution, hematoxylin staining solution, IHC chromogenic reagents, graduated cylinders, humid incubation chambers, slide racks, stainless steel pots or pressure cookers, etc.
Other required reagents & materials not supplied:
Wash buffer, deparaffinization solution, hematoxylin staining solution, IHC chromogenic reagents, graduated cylinders, humid incubation chambers, slide racks, stainless steel pots or pressure cookers, etc.
Storage Conditions and Shelf Life
Storage: Room temperature (2–30 °C); shelf life: 18 months.
Use within 3 months after opening.
Production date and expiry date: see product label.
Use within 3 months after opening.
Production date and expiry date: see product label.
Sample Requirements
Formalin-fixed, paraffin-embedded tissue sections.
Detection Procedure
Ready-to-use without dilution; apply as required.
1.Automated IHC staining system: Pour the buffer into the designated tank of the automated tissue stainer.
2.Manual IHC staining: Perform epitope retrieval following standard protocols.
1.Automated IHC staining system: Pour the buffer into the designated tank of the automated tissue stainer.
2.Manual IHC staining: Perform epitope retrieval following standard protocols.
Result Interpretation
Interpretation depends on tissue type and experimental purposes.
Note: For clinical IHC testing, results must be interpreted by qualified pathologists.
Influencing Factors
1.Operators must receive professional training and follow standard operating procedures.
2.Measuring tools and instruments shall be calibrated to avoid errors.
Note: For clinical IHC testing, results must be interpreted by qualified pathologists.
Influencing Factors
1.Operators must receive professional training and follow standard operating procedures.
2.Measuring tools and instruments shall be calibrated to avoid errors.
Limitations
1.Professional operators and certified laboratories standardize testing and reduce operational deviations caused by external factors.
2.Heat retrieval duration varies greatly due to differences in tissue fixation conditions; users shall optimize conditions via preliminary tests.
2.Heat retrieval duration varies greatly due to differences in tissue fixation conditions; users shall optimize conditions via preliminary tests.
Performance Indicators
1.Appearance & Properties
Clear and complete outer packaging labels; colorless homogeneous liquid reagent.
2.Net volume shall not be lower than the labeled volume.
3.pH Compliance
Measured pH value ranges from 5.5 to 6.5 (at 25±1 °C).
4.Intra-batch Repeatability
pH values of single batch reagents remain 5.5–6.5 (25±1 °C).
5.Inter-batch Repeatability
pH values across different batches stay within 5.5–6.5 (25±1 °C).
Clear and complete outer packaging labels; colorless homogeneous liquid reagent.
2.Net volume shall not be lower than the labeled volume.
3.pH Compliance
Measured pH value ranges from 5.5 to 6.5 (at 25±1 °C).
4.Intra-batch Repeatability
pH values of single batch reagents remain 5.5–6.5 (25±1 °C).
5.Inter-batch Repeatability
pH values across different batches stay within 5.5–6.5 (25±1 °C).
Precautions
1.This reagent is only for epitope retrieval of formalin-fixed paraffin-embedded tissue sections before IHC staining.
2.Read the full manual before experiments.
3.Use within the stated shelf life.
4.Restricted to professionally trained or experienced operators.
5.Over-retrieval may lead to abnormal staining; insufficient retrieval causes weak signals.
6.Optimize retrieval conditions via pre-experiments; use positively charged adhesive slides.
7.Avoid contact with eyes and mucous membranes. Flush exposed areas with abundant clean water immediately if contact occurs.
8.Wear latex gloves, masks, goggles and lab coats for protection during operation.
9.All waste generated shall be disposed of in accordance with Regulations on the Administration of Medical Wastes.
2.Read the full manual before experiments.
3.Use within the stated shelf life.
4.Restricted to professionally trained or experienced operators.
5.Over-retrieval may lead to abnormal staining; insufficient retrieval causes weak signals.
6.Optimize retrieval conditions via pre-experiments; use positively charged adhesive slides.
7.Avoid contact with eyes and mucous membranes. Flush exposed areas with abundant clean water immediately if contact occurs.
8.Wear latex gloves, masks, goggles and lab coats for protection during operation.
9.All waste generated shall be disposed of in accordance with Regulations on the Administration of Medical Wastes.
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