PRPF38A Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:200 |
FC | 1:10 - 1:50 |
Description | Rabbit polyclonal antibody to PRPF38A |
Specificity | Recognizes endogenous levels of PRPF38A protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human PRPF38A. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 37 kD; Observed: 43 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Pre-mRNA-splicing factor 38A |
Gene Symbol | PRPF38A |
Entrez Gene | 84950(Human) |
SwissProt | Q8NAV1(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of PRPF38A expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 43 kD)
Immunohistochemical analysis of PRPF38A staining in human cervix carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HepG2 cells using Anti-PRPF38A Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
FAQs
New Products
