PRDM1 Rabbit Polyclonal Antibody
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6810
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Product Details
Background
Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene . Drives the maturation of B-lymphocytes into Ig secreting cells . Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (By similarity).
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:500 - 1:1000 |
FC | 1:50 - 1:100 |
IHC | 1:100 - 1:500 |
Overview
Product Name | PRDM1 Rabbit Polyclonal Antibody |
Antibody Type | Rabbit Polyclonal Antibody |
Immunogen | Synthetic peptide within Human aa 770-825 / 825 |
Species Reactivity | Human, Mouse, Rat |
Validated Applications | IHC-P, FC, WB |
Molecular Weight | Predicted band size: 92 kDa |
Positive Control | Rat cervix tissue, human skin tissue, human esophagus tissue, mouse esophagus tissue, PANC-1, mouse kidney tissue lysate, rat kidney tissue lysate |
Conjugation | unconjugated |
Form | Liquid |
Form/Buffer | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide |
Isotype | IgG |
Purification | Immunogen affinity purified |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of PRDM1 on different lysates with PRDM1 antibody
Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-PRDM1 antibody.
Flow cytometric analysis of PRDM1 was done on PANC-1 cells.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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