POLD1 Rabbit Monoclonal Antibody(ARA680)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6394
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Product Details
Background
The gene polymerase delta 1 (POLD1) encodes the large, POLD1/p125, catalytic subunit of the DNA polymerase delta (Polδ) complex. The Polδ enzyme is responsible for synthesizing the lagging strand of DNA, and has also been implicated in some activities at the leading strand. The POLD1/p125 subunit encodes both DNA polymerizing and exonuclease domains, which provide the protein an important second function in proofreading to ensure replication accuracy during DNA synthesis, and in a number of types of replication-linked DNA repair following DNA damage. Germline mutations impairing activity of POLD1 have been implicated in several types of hereditary cancer, in some sporadic cancers, and in a developmental syndrome of premature aging, Mandibular hypoplasia, Deafness, and Progeroid features and Lipodystrophy (MDPL/MDP syndrome). Studies of POLD1 emphasize the importance of maintaining genomic stability to limit tumorigenesis. It is currently unclear whether the enhanced tumorigenesis associated with POLD1 defects is the result of increased base substitutions or due to fork collapse and production of DNA double strand breaks (DSBs). Recent reviews have addressed important functions of POLD1 and Polδ.
Application
|
Application |
Dilution Ratio |
|
IHC |
1:200 - 1:1000 |
Overview
|
Product Description |
Recombinant Rabbit monoclonal Antibody to POLD1 |
|
Immunogen |
Synthetic peptide within Human POLD1 aa 1,001-1,050 / 1,107. |
|
Purification Method |
Protein A affinity purified. |
|
Clonality |
Monoclonal |
|
Form |
Liquid |
|
Storage Buffer |
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Isotype |
IgG |
|
Validated Applications |
WB, IHC |
|
Reactivity Species |
Human, Mouse, Rat |
|
Molecular Weight |
Predicted band size: 124 kDa |
|
Positive Control |
HeLa cell lysate, A549 cell lysate, 293T cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, PC-3M cell lysate, mouse testis tissue lysate, rat testis tissue lysate, SW480 cell lysate. |
|
Conjugation |
unconjugated |
Data
Immunohistochemical analysis of paraffin-embedded rat testis tissue with POLD1 Rabbit Monoclonal Antibody(ARA680).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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