PIT-1/POU1F1 Rabbit Monoclonal Antibody(ARA967)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6830
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Size:
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Product Details
Background
PIT-1/POU1F1, known as growth hormone factor-1 (GHF-1), is a member of the POU homeodomain family, and essential for the normal development of the anterior pituitary gland, where it is required to form somatotropes, lactotropes, and thyrotropes.
Transcriptional regulators play a critical role in development by mediating tissue- and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain consisting of two highly conserved regions, tethered by a variable linker of 14-26 amino acids.
In somatotropes and lactotropes, PIT-1 activates the production of growth hormone and prolactin, respectively. In addition, PIT-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated PIT-1a and PIT-1b, which differ in their trans-activation ability.
Mutations in the PIT-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin, and thyroid-stimulating hormone. The gene which encodes PIT-1 maps to human chromosome 3p11.2.
Transcriptional regulators play a critical role in development by mediating tissue- and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain consisting of two highly conserved regions, tethered by a variable linker of 14-26 amino acids.
In somatotropes and lactotropes, PIT-1 activates the production of growth hormone and prolactin, respectively. In addition, PIT-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated PIT-1a and PIT-1b, which differ in their trans-activation ability.
Mutations in the PIT-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin, and thyroid-stimulating hormone. The gene which encodes PIT-1 maps to human chromosome 3p11.2.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Species Reactivity | Humans; others not tested |
Known Applications | Immunohistochemistry (formalin-fixed, paraffin-embedded tissues) |
Supplied As | Buffer with protein carrier and preservative |
Control | Pituitary |
Visualization | Nucleus |
Isotype | IgG |
Immunogen | Recombinant human full-length POU1F1 protein |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human pituitary tissue sections using PIT-1/POU1F1 Rabbit Monoclonal Antibody (ARA967).
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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