OTUD4 Mouse Monoclonal Antibody(C2881)
Datasheet 
Key features and details
Mouse monoclonal to OTUD4
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
CAT.NO. : AMA02493
US$ Please choose
US$ Please choose
Product Details
Background
Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein . May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators . Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
FC | 1:100 - 1:200 |
Overview
Description | Mouse monoclonal to OTUD4 |
Specificity | Recognizes endogenous levels of OTUD4 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human OTUD4 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 124 kD; Observed: 130 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | HIN1; KIAA1046; OTU domain-containing protein 4; HIV-1-induced protein HIN-1 |
Gene Symbol | OTUD4 |
Entrez Gene | 54726(Human) |
SwissProt | Q01804(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of OTUD4 expression in HepG2 (A), C6 (B) whole cell lysates. (Predicted band size: 124 kD; Observed band size: 130 kD kD)
Flow cytometric analysis of HeLa cells using Anti-OTUD4 Antibody (green) and negative control (red).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
New Products
