Oct4 Rabbit Monoclonal Antibody(ARA864)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6690
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
POU5F1 (POU domain, class 5, transcription factor 1), also known as octamer - binding transcription factor-3 oct 3, OCT3), octamer - binding transcription factor-4 (Oct-4, Oct4 and Oct-3/4), modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem - cell self - renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue - specific gene expression in lymphoid and pituitary and in early mammalian development. Oct-3/4 is capable of inducing rapid differentiation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:500-1:4000 |
Overview
Antibody Type | Recombinant Rabbit monoclonal Antibody |
Immunogen | Synthetic peptide within human Oct4 aa 20-60 |
Molecular Weight | Predicted band size: 39 kDa |
Positive Control | F9 cell lysate, NCCIT cell lysate, NCCIT, F9, mouse liver tissue, human seminoma tissue tissue |
Conjugation | unconjugated |
Form/Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol, Preservative: 0.05% Sodium Azide |
Isotype | IgG |
Purification | Protein A affinity purified |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Oct4 Rabbit Monoclonal Antibody(ARA864)
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
New Products
