NSE Mouse Monoclonal Antibody(ARB509)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6801
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
NSE is an intracytoplasmic protein, and enolase is a dimer in mammals. It has three subunits: 1, 2, and 3, which are mainly distributed in central nerve, peripheral nerve, neuroendocrine cells and tumor cells. This antibody can specifically recognize 47kDa of NSE, and can be used for the auxiliary diagnosis of neuroendocrine tumors. Due to the poor specificity of the antibody, some normal smooth muscle, myoepithelial cells, renal tubular cells, lymphocytes can also express NSE. Therefore, this antibody is not used as a single diagnostic marker, and should be applied in combination with other indicators to improve its diagnostic accuracy.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 47kDa |
Purity | ProG affinity purified IgG |
Subcellular location | Cytoplasm |
Swissprot ID | P09104 |
Immunogen | Synthetic peptide within Human NSE aa 416-433. |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
Alternative Names | Neuron-specific enolase, Enolase 2 |
Gene Symbol | ENO2 |
Entrez Gene | 2027 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human pheochromocytoma tissue using NSE Mouse Monoclonal Antibody(ARB509).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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