MTA1 Mouse Monoclonal Antibody(C2857)
MSDS 
Key features and details
Mouse monoclonal to MTA1
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
CAT.NO. : AMA02469
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Product Details
Background
Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator . Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin . In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA . In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A . Acts as a transcriptional coactivator of BCAS3, and SUMO2, independent of the NuRD complex . Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3 . Regulates p53-dependent and -independent DNA repair processes following genotoxic stress .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
FC | 1:100 - 1:200 |
Overview
Description | Mouse monoclonal to MTA1 |
Specificity | Recognizes endogenous levels of MTA1 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human MTA1 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 81 kD; Observed: 80 kD kD |
Form/Buffer | Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Metastasis-associated protein MTA1 |
Gene Symbol | MTA1 |
Entrez Gene | 9112(Human) |
SwissProt | Q13330(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of MTA1 expression in SW480 (A), T47D (B), MCF7 (C), COS7 (D) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 80 kD kD)
Flow cytometric analysis of HeLa cells using Anti-MTA1 Antibody (green) and negative control (red).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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