MSH6 Rabbit Monoclonal Antibody(ARA724)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6511
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Size:
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Product Details
Background
MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI). MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsible for the MSI.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:200-1:1000 |
Overview
Antibody Type | Recombinant Rabbit monoclonal Antibody |
Immunogen | Synthetic peptide within Human MSH6 aa 350-450 (internal sequence). |
Molecular Weight | Predicted band size: 153 kDa |
Positive Control | A549 cell lysate, HepG2 cell lysate, human tonsil tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse small intestine tissue. |
Conjugation | unconjugated |
Form/Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Isotype | IgG |
Purification | Protein A affinity purified. |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with MSH6 Rabbit Monoclonal Antibody(ARA724).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with MSH6 Rabbit Monoclonal Antibody(ARA724).
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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