Mouse GM-CSF/CSF2 ARExSet® antibody pair for ELISA

Protocol Booklet

Mouse GM-CSF/CSF2 ARExSet® antibody pair for ELISA

Key features and details

Species: Mouse
Size: 5 plates,15 plates,50 plates
Sample Volume: 10 μl
Standard Curve Range: 7.81 pg/ml -500 pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 0.02 pg/ml(10 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEM0011

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.1030	0.1051	0.1041
	7.81	0.3055	0.3138	0.3097
0.2056	15.63	0.3873	0.4029	0.3951
0.2911	31.25	0.5245	0.5818	0.5532
0.4491	62.50	0.8136	0.8446	0.8291
0.7251	125.00	1.4340	1.4250	1.4295
1.3255	250.00	2.2960	2.4390	2.3675
2.2635	500.00	3.6660	3.6650	3.6655

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.

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