Mouse Coagulation Factor XII ELISA Kit
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : AEM0041
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Product Details
Background
Coagulation factor XII (F12) is a serum glycoprotein that plays multiple roles in initiating blood coagulation, fibrinolysis, and the production of bradykinin and angiotensin.
typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0164 | 0.0142 | 0.0153 | |
| 13.72 | 0.0253 | 0.0224 | 0.0239 | 0.0086 |
| 41.15 | 0.0286 | 0.0276 | 0.0281 | 0.0128 |
| 123.46 | 0.0472 | 0.0489 | 0.0481 | 0.0328 |
| 370.37 | 0.1131 | 0.1289 | 0.1210 | 0.1057 |
| 1111.11 | 0.3938 | 0.4223 | 0.4081 | 0.3928 |
| 3333.33 | 1.3840 | 1.4040 | 1.3940 | 1.3787 |
| 10000.00 | 3.3980 | 3.3740 | 3.3860 | 3.3707 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average（pg/ml） |
186.2 |
1021.9 |
3453.9 |
143.3 |
846.7 |
2856.7 |
|
Standard deviation |
5.7 |
43.2 |
230.7 |
6.7 |
56.7 |
113.3 |
|
Coefficient of variation（%） |
3.0 |
4.2 |
6.7 |
5.5 |
6.6 |
4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Coagulation Factor XII into health Mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 106% with an overall mean recovery of 100%.
The recovery ranged from 93% to 106% with an overall mean recovery of 100%.
Sample Values
|
Sample Matrix |
Sample Evaluated |
Range (ug/ml) |
Detectable % |
Mean of Detectable (ug/ml) |
|
Serum |
30 |
13.00-60.31 |
100 |
33.76 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Coagulation Factor XII in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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