MLKL Rabbit Monoclonal Antibody(ARA654)

Datasheet

MSDS

Key features and details

Target: MLKL
Host: Rabbit
Reactivity: Human, Mouse, Rat, Chicken
Clonality: Polyclonal
Application: WB, IHC, IF, ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6371

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:2000 - 1:10000
IHC	1:1000 - 1:4000
IF/ICC	1:200 - 1:1000
ELISA	1:2000 - 1:20000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Description	Recombinant Rabbit monoclonal antibody to MLKL
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Clonality	Monoclonal
Isotype	Rabbit / IgG, Kappa
Validated Applications	WB, IHC, IF, ELISA
Reactivity Species	Human, Mouse, Rat, Chicken
Form	Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA
Molecular Weight	55 kDa
Modification/Conjugation	Unmodified
Specificity	Endogenous
Gene Symbol	MLKL
Full Name	Mixed lineage kinase domain-like protein

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with MLKL Rabbit Monoclonal Antibody(ARA654). The HRP-conjugated Rabbit IgG(H + L) Goat antibody was used to detect the antibody.Lane 1: HelaLane 2: U-251 MGLane 3: U-14Lane 4: Rat wombPredicted band size: 55kDaObserved band size: 55kDa

Immunohistochemical staining of human tonsil tissue sections using MLKL Rabbit Monoclonal Antibody (ARA654).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

New Products

Hyaluronic Acid ELISA Kit

Human FADS2 ELISA Kit

AREX® Fluor 488 Rabbit IgG (H&L) Donkey Polyclonal Antibody

AREX® Fluor 647 Mouse IgG (H+L) F(ab')2 Fragment Donkey Polyclonal Antibody

SYN1-Specific Rabbit Monoclonal Antibody(ARA630)

Recombinant Human CD74 Protein