MGMT Mouse Monoclonal Antibody(C3704)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to MGMT |
Specificity | Recognizes endogenous levels of MGMT protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human MGMT. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 21 kD; Observed: 22 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase |
Gene Symbol | MGMT |
Entrez Gene | 4255(Human) |
SwissProt | P16455(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of MGMT expression in Jurkat (A), MCF7 (B), MOLT4 (C), Raji (D) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 22 kD)
Flow cytometric analysis of Jurkat cells using Anti-MGMT Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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