M13 Major Coat Protein Mouse Monoclonal Antibody(ARA828)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6630
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Size:
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Product Details
Background
Morphogenesis of filamentous phage includes synthesis of the phage major coat protein in precursor form, its insertion into the host cell plasma membrane, its cleavage to the mature form of the protein and its assembly there into virions. At each stage of infection, the major coat protein of coliphage M13 binds to the Escherichia coli cytoplasmic membrane with its antigenic site exposed to the cell exterior, which is at the amino - terminus of the protein. Coat protein synthesized in vitro is initially made with a 23 amino acid amino - terminal “leader peptide”, termed “procoat”, that is also a biosynthetic precursor of coat protein in vivo. The filamentous bacteriophage major coat protein occurs as a membrane - spanning assembly intermediate prior to incorporation into the lipid - free virion. Assembly of coliphage M13 is known to occur as the viral DNA crosses the cytoplasmic membrane, shedding its virus - coded DNA unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100 - 1:1000 |
IP | 1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate) |
IF/ICC | 1:50 - 1:500 |
FC | 1 µg per 1 x 10⁶ cells |
Overview
Isotype | IgG2b kappa light chain |
Research Area | Basal & General Transcription, Epitope Tags & General Antibodies |
Target Antigen | M13 major coat protein (g8p) |
Molecular Weight | 5 kDa |
Gene Aliases | M13 bacteriophage g8p, M13 Major Coat Protein B, Gene VIII protein |
Species Reactivity | M13 Bacteriophage |
Immunogen | Filamentous phage M13 |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of M13 Major Coat Protein expression in M13 phage lysate.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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