LRG1 Mouse Monoclonal Antibody (C-5)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA0105
US＄ Please choose
US＄ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
LRG1 (leucine - rich α - 2 - glycoprotein), also known as LRG, is a 347 amino acid secreted protein that contains eight LRR (leucine - rich) repeats and one LRRCT domain. The leucine - rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein - protein interaction, signal transduction, cell adhesion and development. Found mainly in plasma, LRG1 is expressed during granulocyte differentiation. The gene that encodes LRG1 consists of nearly 3,000 bases and maps to human chromosome 19p13.3. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig - like receptors, a number of ICAMs, the CEACAM and PSG families and Fc receptors (FcRs).
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IF/ICC | 1:50-1:500 |
ELISA | 1:30-1:3000 |
IP | 1-2 µg/100-500 µg total protein |
Overview
Isotype | Mouse IgG2b kappa light chain |
Target Antigen | LRG1 (Leucine-rich alpha-2-glycoprotein 1) |
Gene Name | LRG1 |
UniProt ID | P02750 (Human), Q91XL1 (Mouse), Q5M875 (Rat) |
Entrez Gene ID | 116844 (Human), 76905 (Mouse), 497914 (Rat) |
Molecular Weight | 38-50 kDa (due to glycosylation) |
Gene Aliases | LRG, LRG1, Leucine-rich alpha-2-glycoprotein, HMFT1766 |
Immunogen | amino acids 213-257 mapping within an internal region of LRG1 of mouse origin |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of LRG1 expression in non - transfected [unclear text] (A) and mouse LRG1 transfected (B) 293T whole cell lysates.
Western blot analysis of LRG1 expression in mouse plasma tissue extract.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
New Products
