Laminin Receptor Rabbit Monoclonal Antibody(C1783)

Key features and details

Recombinant rabbit monoclonal antibody to Laminin Receptor
  • Target: Laminin Receptor
  • Source/Host: Rabbit
  • Reactivity: Human,Mouse,Rat
  • Clonality: Monoclonal
  • Applications: WB, IP
  • Conjugation: Unconjugated
  • Storage: at-20°C
  • Brand:
CAT.NO. : AMA01395
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Product Details
Background
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB

1:500 - 1:1000

IP

1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview

Description

Recombinant rabbit monoclonal antibody to Laminin Receptor

Specificity

Recognizes endogenous levels of Laminin Receptor protein

Antibody Type

Primary antibody, Recombinant

Imnunogen

KLH-conjugated synthetic peptide encompassing a sequence within human Laminin Receptor protein. The exact sequence is proprietary.

Purification

The antibody was purified by immunogen affinity chromatography.

Molecular Weight

Predicted: 32 kD; Observed: 42 kD

Form/Buffer

Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Alternative Names

LAMBR; LAMR1; 40S ribosomal protein SA; 37 kDa laminin receptor precursor; 37LRP; 37/67 kDa laminin receptor; LRP/LR; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin-binding protein; Laminin receptor 1; LamR; Laminin-binding protein precursor p40; LBP/p40; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4

Gene Symbol

RPSA

Entrez Gene

3921(Human); 102642689;16785(Mouse); 29236(Rat)

SwissProt

P08865(Human); P14206(Mouse); P38983(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data

Western blot analysis of Laminin Receptor expression in PC3 (A), THP1 (B), Hela (C), mouse liver (D), mouse brain (E), rat liver (F), rat brain whole cell lysates. (Predicted band size: 32 kD; Observed band size: 42 kD)

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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