Insulin Rabbit Monoclonal Antibody(ARA985)

Datasheet

MSDS

Insulin Rabbit Monoclonal Antibody(ARA985)

Key features and details

Target: Insulin
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: IF-Cell, IF-Tissue, IHC-P, mIHC, IHC-Fr, WB
Storage: -20°C

Brand:

CAT.NO. : ARA6867

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Insulin (from Latin insula, 'island') is a peptide hormone produced by beta cells of the pancreatic islets; it is considered to be the main anabolic hormone of the body. It regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. In these tissues the absorbed glucose is converted into either glycogen via glycogenesis or fats (triglycerides) via lipogenesis, or, in the case of the liver, into both. Glucose production and secretion by the liver is strongly inhibited by high concentrations of insulin in the blood. Circulating insulin also affects the synthesis of proteins in a wide variety of tissues. It is therefore an anabolic hormone, promoting the conversion of small molecules in the blood into large molecules inside the cells. Low insulin levels in the blood have the opposite effect by promoting widespread catabolism, especially of reserve body fat.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
IF/ICC	1:200 - 1:500
IHC	1:15000 - 1:20000
WB	1:800 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Recombinant protein within human Insulin aa 15-110.
Molecular Weight	Predicted band size: 12 kDa
Positive Control	Mouse pancreas tissue, human pancreas tissue, rat pancreas tissue, Mouse pancreas tissue lysate, Rat pancreas tissue lysate.
Conjugation	unconjugated
Form/Buffer	1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification	Protein A affinity purified.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Insulin on different lysates with Insulin Rabbit Monoclonal Antibody(ARA985) at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Insulin Rabbit Monoclonal Antibody(ARA985) at 1/20,000 dilution.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Insulin Rabbit Monoclonal Antibody(ARA985) at 1/20,000 dilution.

Immunofluorescence analysis of frozen mouse pancreas tissue with Rabbit Insulin Rabbit Monoclonal Antibody(ARA985) at 1/1,000 dilution.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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