Human TNF R Ⅱ/TNFRSF1B ARExSet® antibody pair for ELISA
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
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Brand:
Product Details
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0102 | 0.0103 | 0.0103 | |
| 31.25 | 0.1328 | 0.1373 | 0.1351 | 0.1248 |
| 62.50 | 0.2529 | 0.2655 | 0.2592 | 0.2490 |
| 125.00 | 0.5056 | 0.5203 | 0.5130 | 0.5027 |
| 250.00 | 0.9888 | 0.9038 | 0.9463 | 0.9361 |
| 500.00 | 1.7730 | 1.6570 | 1.7150 | 1.7048 |
| 1000.00 | 2.9280 | 2.6070 | 2.7675 | 2.7573 |
| 2000.00 | 4.0361 | 3.8110 | 3.9236 | 3.9133 |
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
ARExSet®Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
96 well microplates: AREX Biosciences, Catalog # DSEP01.
Plate Sealers: AREX Biosciences, Catalog # DSSF01.
Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.
Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
Wash Buffer: 0.05% Tween 20 in PBS5, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
Assay Buffer: 0.5%BSA, 0.05%Tween20, PBS Solution.AREX Biosciences, Catalog # DSAB01
Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
Stop Solution: 0.5 mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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