Human pTau217 Antibody Pair(ARA980/ARA958)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6854
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Plasma p-tau217 is a biomarker associated with Alzheimer's disease (AD) pathology. Research has shown that plasma p-tau217 is a sensitive marker of the clinical manifestation and progression of AD, including amyloid accumulation, tau burden, brain atrophy, and physical degradation. It has been found to be a potential non-invasive biomarker of AD, with high diagnostic accuracy in differentiating AD from other neurodegenerative diseases. Plasma p-tau217 has also been associated with longitudinal increases in Aβ PET uptake and cognitive decline in Aβ-positive clinically unimpaired individuals. Additionally, higher baseline p-tau217 levels have been linked to greater future tau PET pathology burden and worse future memory performance. These findings suggest that plasma p-tau217 could serve as a minimally invasive diagnostic and prognostic biomarker for AD, with potential utility in clinical practice and trials.
Application
The pTau181 Matched Antibody Pair is expertly designed for immunoassay technologies and high-throughput ELISA platforms that require specifically labeled antibody pairs. These pairs can be customized with a variety of labels such as fluorophores, biotin, and beads. Compatible technologies include MSD, Luminex, Quanterix Simoa, Alpha Technology etc.*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview
Antibody Pair Specificity | pTau217 |
Species Reactivity | Human |
Protein Standard | Recombinant Human p-Tau217 Protein |
Target Antigen | Protein name: Microtubule-associated protein tau; Gene name: MAPT; Organism: Homo sapiens (Human) |
Cross Reactivity | Not Tested |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Sandwich ELISAMicrotiter wells were coated with ARA6846 at 4 µg/mL as the capture antibody. Recombinant pTau217 protein (Cat #: AXP6612) was used as the antigen. 30 ng/mL biotin conjugated Tau Antibody (ARA6807) was used as the detection antibody.Result: ARA6846 and ARA6807 can be used as a matched antibody pair to detect and quantify the concentration of Human pTau217 with high specificity.
Component
| Products include | Catalog No. | Isotype | Quantity | Form |
|---|---|---|---|---|
| pTau217 Antibody | ARA6846 | Mouse IgG1 | 100μg | Liquid |
| Tau Antibody | ARA6807 | Mouse IgG2a | 100μg | Liquid |
| Recombinant Human p-Tau217 Protein | AXP6612 | / | 20μg | Liquid |
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
New Products
