Human pTau205 Antibody Pair(ARA982/ARA971)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA6858
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Product Details
Background
Phosphorylated tau at position 205 (p-tau205) has emerged as a significant biomarker for tau pathology in Alzheimer's disease (AD).Recent studies indicate that p-tau205 levels increase progressively across the AD continuum, correlating strongly with neurofibrillary tangles rather than amyloid plaques, which are typically associated with earlier stages of the disease.This biomarker demonstrates robust associations with tau-PET imaging, particularly in advanced Braak stages of tau pathology, suggesting its potential utility in staging and monitoring disease progression.CSF p-tau205 is also linked to cognitive decline, highlighting its relevance in clinical settings for diagnosing and tracking AD.Its high specificity for tau pathology positions it as a promising alternative to traditional imaging methods in both research and clinical trials aimed at developing AD therapies.
Application
The pTau205 Matched Antibody Pair is expertly designed for immunoassay technologies and high-throughput ELISA platforms that require specifically labeled antibody pairs. These pairs can be customized with a variety of labels such as fluorophores, biotin, and beads. Compatible technologies include MSD, Luminex, Quanterix Simoa, Alpha Technology etc.*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview
Antibody Pair Specificity | pTau205 |
Species Reactivity | Human |
Protein Standard | Recombinant Human p-Tau205 Protein |
Target Antigen | Protein name: Microtubule-associated protein tau; Gene name: MAPT; Organism: Homo sapiens (Human) |
Cross Reactivity | Not Tested |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Sandwich ELISAMicrotiter wells were coated with ARA6848 at 5 µg/mL as the capture antibody. Recombinant pTau205 protein (Cat #: AXP6615) was used as the antigen. 1 µg/mL biotin conjugated Tau Antibody (ARA6837) was used as the detection antibody.Result: ARA6848 and ARA6837 can be used as a matched antibody pair to detect and quantify the concentration of Human pTau205 with high specificity.
Component
| Products include | Catalog No. | Isotype | Quantity | Form |
|---|---|---|---|---|
| pTau205 Antibody | ARA6848 | Mouse IgG1 | 100μg | Liquid |
| Tau Antibody | ARA6837 | Mouse IgG2b | 100μg | Liquid |
| Recombinant Human p-Tau205 Protein | AXP6615 | / | 20μg | Liquid |
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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