Human Prolactin ARExSet® antibody pair for ELISA
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : ASEH0089
US$ Please choose
US$ Please choose
Product Details
Background
Prolactin is a hormone that plays a role in many bodily functions, including:
Lactation: Prolactin is responsible for the production of breast milk in mammals.
Breast development: Prolactin stimulates breast development in women.
Homeostasis: Prolactin is involved in hundreds of actions that help maintain homeostasis.
Stress regulation: Prolactin helps regulate stress.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0430 | 0.0403 | 0.0417 | |
| 78.13 | 0.1258 | 0.1248 | 0.1253 | 0.0837 |
| 156.25 | 0.1903 | 0.2091 | 0.1997 | 0.1581 |
| 312.50 | 0.3603 | 0.3931 | 0.3767 | 0.3351 |
| 625.00 | 0.7231 | 0.7369 | 0.7300 | 0.6884 |
| 1250.00 | 1.5060 | 1.4610 | 1.4835 | 1.4419 |
| 2500.00 | 2.8650 | 2.8400 | 2.8525 | 2.8109 |
| 5000.00 | 4.2603 | 4.3821 | 4.3212 | 4.2796 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . AREX Biosciences, Catalog # DSCB01.
- Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
New Products
