Human IFN-gamma R1 ELISA Kit
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : AEH0104
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Product Details
Background
The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.
typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0285 |
0.0286 |
0.0286 |
|
|
13.72 |
0.0405 |
0.0381 |
0.0393 |
0.0108 |
|
41.15 |
0.0631 |
0.0567 |
0.0599 |
0.0314 |
|
123.46 |
0.1253 |
0.1176 |
0.1215 |
0.0929 |
|
370.37 |
0.3195 |
0.2907 |
0.3051 |
0.2766 |
|
1111.11 |
0.8572 |
0.7749 |
0.8161 |
0.7875 |
|
3333.33 |
2.1650 |
2.0410 |
2.1030 |
2.0745 |
|
10000.00 |
4.0214 |
3.8750 |
3.9482 |
3.9197 |
precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average（pg/ml） |
216.4 |
1044.3 |
3466.9 |
188.9 |
972.7 |
3239.0 |
|
Standard deviation |
9.7 |
50.3 |
148.6 |
15.9 |
64.1 |
77.7 |
|
Coefficient of variation（%） |
4.5 |
4.8 |
4.3 |
4.4 |
6.6 |
2.4 |
- Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
- Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
spike recovery
The spike recovery was evaluated by spiking 3 levels of human IFN gamma R1 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 93% to 120% with an overall mean recovery of 103%.
sample values
|
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable % |
Mean of Detectable (pg/ml) |
|
Serum |
30 |
284.50-993.11 |
100 |
576.85 |
Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human IFN gamma R1 in this assay. No medical histories were available for the donors.
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