Human FOLR1 ELISA

Protocol Booklet

Key features and details

Species: Human
Size: 5 plates,15 plates,50 plates
Sample Volume: 50 μl
Standard Curve Range: 46.88 pg/ml -3000 pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 1.86 pg/ml(50 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEH0137

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Folate receptor α (FRα), also known as folate receptor 1 (FOLR1), belongs to the folate receptor family and are both cysteine-rich glycolipid anchor proteins. FRα is encoded by FOLR1 and is a cell surface glycoprotein with a molecular weight of 38-40KD. It was initially discovered as a folic acid-binding protein. It was later found that in addition to transporting folic acid, it is also involved in regulating the proliferation and metastasis of tumor cells. Folic acid is an important nutrient necessary for maintaining cell growth and metabolism, and it plays a key role in the synthesis of DNA and RNA.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0172	0.0165	0.0169
	46.88	0.0579	0.0669	0.0624
0.0456	93.75	0.1303	0.1231	0.1267
0.1099	187.50	0.2263	0.2564	0.2414
0.2245	375.00	0.5634	0.4782	0.5208
0.5040	750.00	1.1860	1.2520	1.2190
1.2022	1500.00	2.8620	2.9120	2.8870
2.8702	3000.00	4.3398	4.2738	4.3068

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.

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