Human CP(Ceruloplasmin) ELISA Kit
Key features and details
- Sensitivity:75pg/ml
- Detection Range:125-8000pg/ml
- Sample Types: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Species:Human
- Detection Method: Sandwich ELISA, Double Antibody
- Target:CP(Ceruloplasmin)
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Brand:
CAT.NO. : ARE6613
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Size:
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Product Details
Background
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
Overview
|
Alias |
Ceruloplasmin ELISA Kit, Ferroxidase ELISA Kit, CP ELISA Kit |
|
Size |
96T |
|
Species |
Human |
|
UniProt ID |
P00450 |
|
Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
|
Detection Method |
Sandwich ELISA, Double Antibody |
|
Detection Wavelength |
OD450 |
|
Reaction Duration |
4 hours |
|
Range |
125-8000pg/ml |
|
Sensitivity |
75pg/ml |
|
Specificity |
Specifically binds with CP, no obvious cross reaction with other analogues. |
Data
Standard Curve
Storage
Shipped and store at 4°C, don't cryopreserve.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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