Human Complemenet C5 ARExSet® antibody pair for ELISA
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : ASEH0108
US$ Please choose
US$ Please choose
Product Details
Background
Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain. Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure. In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0245 |
0.0211 |
0.0228 |
|
|
31.25 |
0.1362 |
0.1543 |
0.1453 |
0.1225 |
|
62.50 |
0.2475 |
0.2539 |
0.2507 |
0.2279 |
|
125.00 |
0.4582 |
0.4574 |
0.4578 |
0.4350 |
|
250.00 |
0.8325 |
0.8044 |
0.8185 |
0.7957 |
|
500.00 |
1.5790 |
1.4280 |
1.5035 |
1.4807 |
|
1000.00 |
2.6240 |
2.6030 |
2.6135 |
2.5907 |
|
2000.00 |
4.0206 |
4.0585 |
4.0396 |
4.0168 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time.
- Development protocols are provided to guide further assay optimization.
- Economical alternative to complete kits.
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi
ltered . AREX Biosciences, Catalog # DSCB01. - Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution. AREX Biosciences, Catalog # DSAB01
Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01. - Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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