Human Beta Amyloid 1-42 antibody pair (ARA834/ARA954)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6878
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Size:
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Product Details
Background
The Aβ1-42 peptide is a 42-amino acid fragment cleaved from the amyloid precursor protein (APP) by secretase enzymes. It is the longer and more amyloidogenic form of the Aβ peptide, compared to the more common Aβ1-40 form. Aβ1-42 has a greater tendency to aggregate and form amyloid fibrils and plaques in the brain, which is a hallmark pathological feature of Alzheimer's disease. Increased production and accumulation of Aβ1-42 relative to Aβ1-40 is associated with genetic mutations, certain ApoE alleles, and other as-yet undiscovered factors that increase the risk of Alzheimer's disease. Measurement of the Aβ1-42 peptide in cerebrospinal fluid (CSF) is used as a biomarker for Alzheimer's disease, as decreased CSF Aβ1-42 levels indicate increased brain deposition of the peptide.The Aβ1-42/Aβ1-40 ratio in CSF is considered a more sensitive and specific marker of Alzheimer's pathology than Aβ1-42 alone.
Application
The Beta Amyloid 1-40 Matched Antibody Pair is expertly designed for immunoassay technologies and high-throughput ELISA platforms that require specifically labeled antibody pairs. These pairs can be customized with a variety of labels such as fluorophores, biotin, and beads. Compatible technologies include MSD, Luminex, Quanterix Simoa, Alpha Technology etc.*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview
Antibody Pair Specificity | Beta Amyloid 1-42 |
Species Reactivity | Human |
Protein Standard | Human beta Amyloid 1-42 polypeptide |
Target Antigen | Protein name: amyloid beta precursor protein; Gene name: APP; UniProt Accession: P05067; Organism: Homo sapiens (Human) |
Cross Reactivity | Not Tested |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Sandwich ELISAMicrotiter wells were coated with ARA6655 at 3 µg/mL as the capture antibody. Recommbinant Beta Amyloid 1-42 protein was used as the antigen. 1 µg/mL Beta Amyloid Antibody ARA6798 was used as the detection antibody.
Component
| Products include | Catalog No. | Isotype | Quantity | Form |
|---|---|---|---|---|
| Beta Amyloid 1-42 Antibody (ARA835) | ARA6655 | Mouse IgG2a | 100μg | Liquid |
| Beta Amyloid Antibody (ARA954) | ARA6798 | Mouse IgG2a | 100μg | Liquid |
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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