Histone H2A.X Rabbit Polyclonal Antibody

Datasheet

MSDS

Histone H2A.X Rabbit Polyclonal Antibody

Key features and details

Target: Histone H2A.X
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Application: WB, IH, IF/IC, ChIP
Storage: -20°C

Brand:

CAT.NO. : ARA0200

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Histone H2A.X is a variant of the histone H2A protein, and its phosphorylated form, γH2AX (S139), is a key marker for DNA double-strand breaks (DSBs). It plays a crucial role in cell cycle checkpoint activation and DNA repair following DSBs.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:500 - 1:1000
IHC	1:50 - 1:100
IF/ICC	1:50 - 1:200
ChIP	1:10 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Description	Rabbit polyclonal antibody to Histone H2A.X
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone H2A.X. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Form	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Gene Symbol	H2AFX
Alternative Names	H2AX; Histone H2AX; H2a/x; Histone H2A.X
Gene ID (Human)	3014
Protein ID (Human)	P16104

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Histone H2A.X expression in HEK293T (A), Hela (B), mouse testis (C), rat lung (D), rat testis (E) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Immunohistochemical analysis of Histone H2A.X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of Histone H2A.X expression in HEK293T (A), Hela (B), mouse testis (C), rat lung (D), rat testis (E) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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