His-tag Rabbit Polyclonal Antibody
Datasheet 
Key features and details
Rabbit polyclonal antibody to His-tag
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
CAT.NO. : APA12336
US$ Please choose
US$ Please choose
Product Details
Background
The His-tag (polyhistidine-tag) is an amino acid motif consisting of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is widely used for affinity purification of recombinant proteins via immobilized metal affinity chromatography (IMAC) using nickel or cobalt resins. Anti-His antibodies recognize the polyhistidine tag in WB, IP, IHC, IF, and FACS.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:1000 - 1:2000 |
IP | 1:10 - 1:100 |
Overview
Description | Rabbit polyclonal antibody to His-tag |
Specificity | Recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
Antibody Type | Primary antibody,Tag |
Imnunogen | KLH-conjugated synthetic peptide HHHHHH. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | N/A |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag |
Gene Symbol | |
Entrez Gene | |
SwissProt |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of Anti-His-tag Antibody against HEK293T cells transfected with vector overexpressing His tag (A) and untransfected (B).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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