HAUS7 Rabbit Monoclonal Antibody(C1030)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to HAUS7 |
Specificity | Recognizes endogenous levels of HAUS7 protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human HAUS7. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 39 kD; Observed: 37 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Names | UCHL5IP; UIP1; HAUS augmin-like complex subunit 7; 26S proteasome-associated UCH37-interacting protein 1; UCHL5-interacting protein; X-linked protein STS1769 |
Gene Symbol | HAUS7 |
Entrez Gene | 55559(Human); 73738(Mouse) |
SwissProt | Q99871(Human); Q8BKT8(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of HAUS7 expression in HEK293T (A), Jurkat (B), A549 (C), mouse kidney (D) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 37 kD)
Immunofluorescent analysis of HAUS7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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