GPR41 Rabbit Polyclonal Antibody
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Gene Symbol:
- Storage:
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Brand:
CAT.NO. : APA07044
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Product Details
Background
G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IF/ICC |
1:50 - 1:200 |
Overview
|
Product Description |
Rabbit polyclonal antibody to GPR41 |
|
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR41. The exact sequence is proprietary. |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Polyclonal |
|
Form |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
|
Gene Symbol |
FFAR3 |
|
Alternative Names |
GPR41; Free fatty acid receptor 3; G-protein coupled receptor 41 |
|
Gene ID (Human) |
2865 |
|
Gene ID (Mouse) |
233080 |
|
Protein ID (Human) |
O14843 |
|
Protein ID (Mouse) |
Q3UFD7 |
Data
Western blot analysis of GPR41 expression in rat liver (A) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 39 kD)
Immunofluorescent analysis of GPR41 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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