GPER Rabbit Monoclonal Antibody(C4063)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
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Brand:
Product Details
|
Application |
Dilution Ratio |
|
WB |
1:500-1:1000 |
|
IHC |
1:50-1:200 |
|
IF/ICC |
1:50-1:200 |
|
Product Description |
Recombinant rabbit monoclonal antibody to GPER |
|
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within human GPER protein. The exact sequence is proprietary. |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Monoclonal |
|
Form |
Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300. |
|
Gene Symbol |
GPER1 |
|
Alternative Names |
CEPR; CMKRL2; DRY12; GPER; GPR30; G-protein coupled estrogen receptor 1; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; G protein-coupled estrogen receptor 1; G-protein coupled receptor 30; GPCR-Br; IL8-related receptor DRY12; Lymphocyte-derived G-protein coupled receptor; LYGPR; Membrane estrogen receptor; mER |
|
Gene ID (Human) |
2852 |
|
Gene ID (Mouse) |
76854 |
|
Gene ID (Rat) |
171104 |
|
Protein ID (Human) |
Q99527 |
|
Protein ID (Mouse) |
Q8BMP4 |
|
Protein ID (Rat) |
O08878 |
Western blot analysis of GPER expression in mouse brain (A) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 55 kD)
Immunohistochemical analysis of GPER staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GPER staining in ASPC1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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