FTO Rabbit Monoclonal Antibody(C3152)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:100 |
IF/ICC | 1:50 - 1:100 |
Description | Rabbit monoclonal antibody to FTO |
Specificity | Recognizes endogenous levels of FTO protein. |
Antibody Type | Primary antibody |
Imnunogen | A synthetic peptide of human FTO |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 58 kD; Observed: 58 kD |
Form/Buffer | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
Alternative Names | KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein |
Gene Symbol | FTO |
Entrez Gene | 79068(Human) |
SwissProt | Q9C0B1(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of FTO expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)
Immunohistochemical analysis of FTO staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FTO staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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