ERK2 Rabbit Monoclonal Antibody(ARA773)

Datasheet

MSDS

Key features and details

Target: ERK2
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Applications: WB,IHC,IP,FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6560

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000-1:2000
IHC	1:800-1:1600
FC	1:200-1:1000
IP	1:20

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to ERK2
Antibody Type	Primary antibody
Predicted MW	41kDa
Immunogen	A synthetic peptide corresponding to the C-term of ERK2 was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.48%.
Alternative Names	MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1
Gene Symbol	MAPK1; MAPK3
Entrez Gene	5594; 5595(Human)
Swissprot	P28482

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-ERK2 antibody at 1:1,000 dilution
Predicted MW: 41 kDa
Observed MW: 42 kDa

Lane 1: JurKat
Lane 2: Hela
Lane 3: 293
Lane 4: A431
Lane 5: Raw264.7
Lane 6: 3T3
Lane 7: PC-12

Lysate at 10 µg per lane
2nd Ab:

All lanes: Anti-ERK2 antibody at 1:1,000 dilution
Predicted MW: 41 kDa
Observed MW: 42 kDa

Lane 1: Mu Brain
Lane 2: Mu Heart
Lane 3: Mu Kidney
Lane 4: Mu Liver
Lane 5: Rat Heart
Lane 6: Rat Kidney
Lane 7: Rat Liver

Lysate at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:10,000

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium cancer tissue labelling ERK2 with ARA773 at 1:1,600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Anti-ERK2 was immunoprecipitated from 0.4mg of A431 lysate with ARA773 at 1:20 dilution.
2nd Ab:
GAR HRP for IP 1:10,000
Lane 1: ARA773 IP in A431 whole cell lysate
Lane 2: PBS instead of ARA773 in A431 whole cell lysate Lane3: A431 whole cell lysate, 10 µg(input)
Exposure: 120s

Overlay histogram showing Hela cells stained with ARA773 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA773 , 1:200 dilution) in 1x PBS/1% BSA for 30 min at room temperature . The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature . Unlabelled sample (Black) was used as a control.

ARA773 staining ERK2 in Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:50) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(1:500).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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