Desmin Mouse Monoclonal Antibody(ARB942)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6734
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Product Details
Background
Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas.Desmin is a 53kDa intermediate filament (IF) protein that integrates the sarcolemma, Z disk, and nuclear membrane in sarcomeres and regulates sarcomere architecture. Desmin play no role in contractility but serves to maintain the orientation of actin and myosin filaments and may also play a role in nuclear transcription.Desmin is expressed in all striated muscle cells and most smooth muscle cells. In the developing striated muscle cells it replaces vimentin, linked to the Z - discs. In smooth muscle cells desmin is associated with cytoplasmic dense bodies and subplasmalemmal dense plaques. In myofibroblasts and vascular smooth muscle cells desmin co - expressed with vimentin. However, in the arterial smooth muscle cells, desmin may be scarce or absent, while vimentin expression is retained. Mesothelial cells may express desmin, particularly in effusions and cultures. In tumor tissues, it detected in leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, paracardial, and perivascular cells of glomus tumors of the skin.Desmin is used in a panel for identification of leiomyosarcoma, rhabdomyosarcoma and other tumours with myoid differentiation, and for classification of mesenchymal, pleomorphic, spindle cell and round cell neoplasms.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 53kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Cytoplasm |
Swissprot ID | P17661 |
Immunogen | Immunogen derived from human Leiomyoma |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
Alternative Names | Desmin |
Gene Symbol | DES |
Entrez Gene | 1674 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human Leiomyosarcoma tissue using Desmin Mouse Monoclonal Antibody(ARB942).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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