Claudin 4 Mouse Monoclonal Antibody(ARA845)

Datasheet

MSDS

Claudin 4 Mouse Monoclonal Antibody(ARA845)

Key features and details

Target: Claudin 4
Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Application: IHC
Storage: -20°C

Brand:

CAT.NO. : ARA6669

US$ Please choose

Size:

1mL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl^- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
IHC	1:100 - 1:300

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Immunogen	KLH - conjugated synthetic peptide encompassing a sequence within human Claudin 4. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Clone Type	Monoclonal
Form/Buffer	Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Gene Name	CLDN4
Related Names	CPER; CPETR1; WBSCR8; Claudin - 4; Clostridium perfringens enterotoxin receptor; CPE - R; CPE - receptor; Williams - Beuren syndrome chromosomal region 8 protein
Gene ID (Human)	1364
Protein ID (Human)	O14493

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of Claudin 4 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Claudin 4 staining in human pancreatic carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?

AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.

What development scenarios are pathology raw antibodies mainly used for?

Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.

How can I evaluate whether a raw antibody is suitable for our staining platform?

It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.

Can you provide samples for platform validation?

Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.

How should raw antibodies be paired with detection systems?

AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.

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