Clathrin Heavy Chain Rabbit Polyclonal Antibody

Datasheet

MSDS

Clathrin Heavy Chain Rabbit Polyclonal Antibody

Key features and details

Rabbit polyclonal antibody to Clathrin Heavy Chain

Target: Clathrin Heavy Chain
Source/Host: Rabbit
Reactivity: Human,Mouse,Rat
Clonality: Polyclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA13384

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge . The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension . Plays a role in early autophagosome formation . Interaction with DNAJC6 mediates the recruitment of HSPA8 to the clathrin lattice and creates local destabilization of the lattice promoting uncoating .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to Clathrin Heavy Chain
Specificity	Recognizes endogenous levels of Clathrin Heavy Chain protein.
Antibody Type	Primary antibody
Imnunogen	KLH-conjugated synthetic peptide of human Clathrin Heavy Chain
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 187; Observed: 191 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	CLH17; CLTCL2; KIAA0034; Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; CLH-17
Gene Symbol	CLTC
Entrez Gene	1213(Human); 67300(Mouse); 54241(Rat)
SwissProt	Q00610(Human); Q68FD5(Mouse); P11442(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Clathrin Heavy Chain expression in Hela (A), MCF7 (B), mouse lung (C), rat brain (D) whole cell lysates. (Predicted band size: 187; 191 kD; Observed band size: 191 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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