CD4 Rabbit Monoclonal Antibody(ARB909)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6701
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Size:
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Product Details
Background
The CD4 molecule is a glycoprotein, belonging to immunoglobulin superfamily that is associated with HLA class II antigen recognition. It is a co - receptor that assists the T - cell receptor (TCR) with an antigen - presenting cell and also interacts directly with MHC class II molecules on the surface of the antigen - presenting cells using its extracellular domain. In lymphatic tissues, the CD4+ T cells are seen in large numbers in the parafollicular zone, while scattered cells are found in the germinal centres and mantle zone. CD4 is also demonstrated in hepatic sinusoidal cells, monocytes and monocytes - derived cells but not expressed on B cells and immature thymocytes. Most mature T - cell lymphomas are CD4 positive with the exception of aggressive NK - cell leukaemia and extranodal NK/T - cell lymphomas: subcutaneous panniculitis - like T - cell lymphoma (which is usually CD8 positive), enteropathy - type T - cell lymphoma and hepatosplenic T - cell lymphoma. CD4 is also expressed in histiocytic sarcomas and Langerhans cell histiocytosis as well as in splenic littoral cell haemangioma. CD4 plays an important role in the classification of lymphocytes in inflammatory lesions and malignant lymphomas.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 51 kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Membrane |
Swissprot ID | P01730 |
Immunogen | Synthetic peptide corresponding to CD4 residues within aa200-300 |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18° C-25° C) for 30 minutes. |
Alternative Names | T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4 |
Gene Symbol | CD4 |
Entrez Gene | 920 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human T Cell Lymphoma tissue sections using CD4 Rabbit Monoclonal Antibody (ARB909).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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