CD25 Rabbit Monoclonal Antibody(ARM945)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARM6735
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Cluster of Differentiation 25 (CD25) is the extracellular region of the interleukin-2 receptor on activated B- and T-cells, myeloid precursors, and some other types of leukocytes. Since CD25 staining is useful for differentiating neoplastic mast cell aggregates from reactive proliferations, Anti-CD25 has become a major criterion for Systemic Mastocytosis diagnoses. In the context of urticaria pigmentosa, CD25 staining is also used to recognize mast cells in skin biopsies. There is a greater proportion of CD25+ and FOXP3+ regulatory T-cells among cancer cells in tumour parenchyma and its periphery in recurrent cutaneous melanoma compared to non-recurrent melanoma.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | IHC |
Dilution Ratio | 1:50 - 1:200 |
Overview
Antibody Type | Primary antibodies |
Isotype | IgG |
Positive Control | Lesions of Mastocytosis |
Localization | Cytoplasmic, Membranous |
Form/Buffer | Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide |
Purification | Purified |
Conjugation | Unconjugated |
Alternative Names | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD antigen CD25 |
Gene Symbol | IL2RA |
Entrez Gene | 3559 |
Uniprot | P01589 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human spleen tissue using CD25 Rabbit Monoclonal Antibody (ARM945).
Research Use Only
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
New Products
