Anti-H3N8 Avian Influenza HA Protein Mouse Monoclonal Antibody (H3_4C2)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : AXA0003
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Size:
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Product Details
Background
The H3N8 Avian Influenza HA Protein (Hemagglutinin Protein) is a pivotal surface glycoprotein of the H3N8 avian influenza virus, playing a decisive role in viral entry into host cells. As a class I fusion protein, it exists as a stable homotrimer, with each monomer consisting of two functional subunits (HA1 and HA2) linked by disulfide bonds. The HA1 subunit contains the receptor-binding site (RBS), which specifically recognizes and binds to sialic acid receptors on the surface of host epithelial cells—this binding is the initial step of viral attachment. The HA2 subunit, anchored in the viral envelope, mediates the fusion of the viral membrane with the host cell endosomal membrane through pH-induced conformational changes, allowing the viral genome to enter the host cytoplasm for replication.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:200:1:1000 |
IF/ICC | 1:50-1:500 |
Hemagglutination Inhibition | 1:1000-1:5120 |
Overview
Description | Mouse Monoclonal antibody to H3N8 Avian Influenza HA Protein |
Antibody Type | Primary antibody |
Immunogen | H3N8 Avian Influenza Virus HA Protein |
Form/Buffer | PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
Alternative Names | H3N8 HA, H3 hemagglutinin, Avian influenza H3 HA, AIV H3N8 hemagglutinin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Results from WB and IFA show that this antibody can effectively recognize the H3N8 HA protein expressed in 293T cells.
Results from WB and IFA show that this antibody can effectively recognize the H3N8 HA protein expressed in 293T cells.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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