Anti-Feline Calicivirus (FCV) VP1 Protein Mouse Monoclonal Antibody (3C10)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : AXA0044
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Size:
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Product Details
Background
Feline Calicivirus (FCV) VP1 Protein is the major capsid protein of FCV, a non-enveloped single-stranded RNA virus belonging to the Caliciviridae family that causes highly contagious respiratory and oral diseases in cats. As the most abundant structural protein of the virus, accounting for nearly 90% of the viral capsid composition, VP1 is encoded by the viral open reading frame 2 (ORF2) and has a molecular weight of approximately 70 kDa, with a structure consisting of conserved regions and variable domains that contribute to its multiple biological functions. The VP1 protein self-assembles into an icosahedral capsid that encapsulates the viral genome, providing structural stability and protecting the genetic material from environmental degradation while mediating the initial interaction with host cells—specifically, its N-terminal and C-terminal regions bind to surface receptors on feline epithelial cells, facilitating viral entry through receptor-mediated endocytosis.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB((Only GI-type virus response)) | 1:200-1:1000 |
IF/ICC | 1:500-1:1000 |
Neutralization | 1:10-1:200 |
Overview
Description | Mouse Monoclonal antibody to Feline Calicivirus (FCV) VP1 Protein |
Antibody Type | Primary antibody |
Isotype | IgG2a |
Immunogen | FCV Whole Virus |
Form/Buffer | PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
Alternative Names | FCV VP1, Feline calicivirus capsid protein, FCV major capsid protein VP1 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
IFA results indicate that this antibody can effectively recognize FCV - infected cells.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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