Anti-Canine Coronavirus (CCoV) M Protein Mouse Monoclonal Antibody (2H11)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : AXA0030
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Canine Coronavirus (CCoV) M Protein is a key membrane glycoprotein of CCoV, localized on the viral envelope. It plays multiple critical roles in the viral life cycle, including mediating viral assembly, budding, and membrane fusion with host cells. As an important immunogenic protein, it can induce the host to produce specific antibodies and serves as a target for the development of CCoV diagnostic reagents and vaccines. Its structural characteristics are closely related to the virus's pathogenicity and interspecies transmission potential.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:200-1:1000 |
IF/ICC | 1:500-1:1000 |
ELISA | 1:2000-1:10000 |
Overview
Description | Mouse Monoclonal antibody to Canine Coronavirus (CCoV) M Protein |
Antibody Type | Primary antibody |
Isotype | IgG3 |
Immunogen | CCoV Whole Virus |
Form/Buffer | PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
Alternative Names | CCoV M, Canine coronavirus membrane protein, CCoV matrix glycoprotein, CCoV M glycoprotein |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
The WB results showed that this antibody can effectively recognize the CCoV M protein.
The IFA results indicated that this antibody can effectively recognize CCoV-infected cells.
Reference
甘军纪，吕海峰，汤也，田晓彦，刘秀梵. 犬冠状病毒M和N蛋白单克隆抗体的制备及其生物学特性鉴定,中国兽医学报,2021, 41(8),1482-1489.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
New Products
