ALKBH3 Rabbit Polyclonal Antibody
Datasheet 
Key features and details
Rabbit polyclonal antibody to ALKBH3
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : APA13299
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Product Details
Background
Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) . Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation . Has a strong preference for single-stranded DNA . Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 . Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA . Also acts on RNA . Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons . Requires molecular oxygen, alpha-ketoglutarate and iron .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:2000 |
Overview
Description | Rabbit polyclonal antibody to ALKBH3 |
Specificity | Recognizes endogenous levels of ALKBH3 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human ALKBH3 |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 33 kD; Observed: 36 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | ABH3; DEPC1; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; Alkylated DNA repair protein alkB homolog 3; DEPC-1; Prostate cancer antigen 1 |
Gene Symbol | ALKBH3 |
Entrez Gene | 221120(Human); 69113(Mouse); 362169(Rat) |
SwissProt | Q96Q83(Human); Q8K1E6(Mouse); Q5XIC8(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of ALKBH3 expression in HepG2 (A), Hela (B), MCF7 (C) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 36 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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