Actin Rabbit Monoclonal Antibody(ARA776)

Datasheet

MSDS

Actin Rabbit Monoclonal Antibody(ARA776)

Key features and details

Target: Actin
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat, Dog, Bovine, Pig, Chicken,
Clonality: Monoclonal
Applications: WB,IHC,IF/ICC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6563

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. This antibody is predicted to react with all actin isoforms, including: 1) sarcomeric actins, α-skeletal (α-SKA) and α-cardiac (α -CAA), 2) smooth muscle actins (SMAs), α-SMA and γ-SMA, and 3) cytoplasmic actins (CYAs), β-CYA and γ-CYA.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000-1:2000
IHC	1:800-1:1600
IF/ICC	1:10-1:50
IP	1:40

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to Actin
Antibody Type	Primary antibody
Predicted MW	42kDa
Immunogen	A synthetic peptide corresponding to the N-term of actin was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.51%.
Alternative Names	ACTA; Actin alpha skeletal muscle; Alpha-actin-1; ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin
Gene Symbol	ACTA1; ACTC1
Entrez Gene	58; 70(Human)
Swissprot	P68133

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-actin antibody at 1:1,000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa

Lane 1: Jurkat
Lane 2: Hela
Lane 3: 293T
Lane 4: Raw264.7
Lane 5: A431
Lane 6: A375
Lane 7: 3T3

Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s

All lanes: Anti-actin antibody at 1:1,000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa

Lane 1: MDBK
Lane 2: MDCK
Lane 3: COS-7
Lane 4: Pig heart
Lane 5: Chicken heart
Lane 6: African clawed frog
Lane 7: Zebrafish

Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s

All lanes: Anti-actin antibody at 1:1,000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa

Lane 1: Mouse brain
Lane 2: Mouse heart
Lane 3: Mouse kidney
Lane 4: Mouse liver
Lane 5: Rat brain
Lane 6: Rat liver
Lane 7: Rat kidney
Lane 8: Rat liver

Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic adenocarcinoma tissue labelling actin with ARA776 at 1:1,600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

ARA776 staining Actin in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:50) at 4°C. An Alexa Fluor<sup>®</sup> 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An Alexa Fluor<sup>®</sup> 488-conjugated Goat Anti-Rabbit IgG (1:500).

Actin was immunoprecipitated from 0.4mg of PC-12 whole cell lysate with ARA776 at 1:40 dilution.
2nd Ab:
GAR HRP for IP 1:500

Lane 1: ARA776 IP in PC-12 whole cell lysate
Lane 2: PBS instead of ARA776 in PC-12 whole cell lysate
Lane 3: PC-12 whole cell lysate, 10 µg (input)

Exposure: 30s

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

New Products

Hyaluronic Acid ELISA Kit

Human FADS2 ELISA Kit

AREX® Fluor 488 Rabbit IgG (H&L) Donkey Polyclonal Antibody

AREX® Fluor 647 Mouse IgG (H+L) F(ab')2 Fragment Donkey Polyclonal Antibody

SYN1-Specific Rabbit Monoclonal Antibody(ARA630)

Recombinant Human CD74 Protein